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Biology & Genetics Tools

Free biology and genetics calculators that run entirely in your browser — Punnett squares, DNA↔RNA transcription, codon-to-protein translation, GC content, reverse complement, Hardy-Weinberg equilibrium, primer melting temperature, cell doubling time, CFU counts and molecular weight. Nothing is uploaded.

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Punnett Square Calculator

Cross two genotypes to build a Punnett square with genotype and phenotype ratios for monohybrid and dihybrid crosses.

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DNA ⇄ RNA Transcription Converter

Transcribe a DNA coding or template strand into mRNA, or reverse-transcribe RNA to DNA, with length and base composition.

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Codon → Amino Acid Translator

Translate a DNA or RNA sequence into a protein using the standard genetic code, with reading frame, start codon and 1- or 3-letter output.

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GC Content Calculator

Compute GC and AT content, base counts, GC skew and optional sliding-window local GC% for a DNA or RNA sequence.

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Reverse Complement Calculator

Get the reverse complement, complement and reverse of a DNA or RNA sequence, with IUPAC ambiguity codes supported.

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Hardy-Weinberg Equilibrium Calculator

Compute allele frequencies p and q, expected genotype frequencies and a chi-square test for Hardy-Weinberg equilibrium.

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Primer Melting Temperature (Tm) Calculator

Estimate PCR primer melting temperature with the Wallace rule or a salt-adjusted formula, plus GC% and annealing temperature.

Cell Doubling / Generation Time Calculator

Find bacterial doubling time, specific growth rate, or final cell count from an initial and final count over an elapsed time.

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CFU/mL Colony Count Calculator

Convert plate colony counts and dilution factor into CFU/mL, with log10 titre and a 30-300 countable-range check.

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DNA / Oligo Molecular Weight Calculator

Compute molecular weight of a DNA, dsDNA, or RNA sequence from base composition, with length, GC%, and nmol↔µg conversion.

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Protein Molecular Weight & pI Calculator

Compute a protein's molecular weight in Da/kDa and its isoelectric point (pI) from a one-letter amino-acid sequence.

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OD600 to Cells/mL Converter

Convert an OD600 reading to cells per mL using an organism-specific factor, with dilution, total cells, and target-OD dilution.

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Microscope Magnification & Field of View Calculator

Compute total microscope magnification and field of view diameter from objective, eyepiece, and field number.

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