About this tool
Plan a serial dilution and get the concentration of every tube plus the exact transfer and diluent volumes needed. Enter the stock concentration, the dilution factor, how many steps and the total volume per tube. All calculations run in your browser.
Each step divides the concentration by the dilution factor, so the concentration at tube n is Cn = C0 / DF^n. For a fixed total volume per tube, the transfer volume is total volume / DF and the diluent volume is total volume − transfer volume. For example a 10-fold dilution into 1 mL tubes means moving 0.1 mL and adding 0.9 mL of diluent at each step.
The results table lists every tube from the undiluted stock (tube 0) through the final dilution, showing the dilution ratio (1:DF^n), the concentration and the per-tube transfer and diluent volumes. It is ideal for preparing standard curves, plating assays and titre determinations.